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L2785
Sigma
Lectin from Dolichos biflorus (horse gram)
lyophilized powder
| Synonym: | Dolichos biflorus agglutinin, DBA |
Description
| Analysis Note | Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A1 erythrocytes after 1 hour incubation at 25 °C. |
| Biochem/physiol Actions | DBA has anti-A1 human blood group specificity, useful for distinguishing between A1 and A2 blood types. DBA has strong anti-Cad activity,1 and has an affinity for terminal N-acetyl-α- |
| Preparation Note | Purified by affinity chromatography. |
Properties
| form | lyophilized powder |
| potency | <20 μg per mL agglutination activity |
| total impurities | salt, essentially free |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| Cited Reference | 1. Bird, G. W. G. Blood 21, 366, (1970) |
| 2. Etzler, M. E., Kabat, E. A. Biochemistry 9, 869, (1970) | |
| reference | Etzler, M. E. Meth. Enzymol. 28, 340, (1972) |
